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3d images analyses  (Oxford Instruments)


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    Oxford Instruments 3d images analyses
    The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial <t>volume</t> <t>Imaris</t> iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. <t>3D</t> images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.
    3d Images Analyses, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 43547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3d images analyses - by Bioz Stars, 2026-07
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    1) Product Images from "Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission"

    Article Title: Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission

    Journal: iScience

    doi: 10.1016/j.isci.2024.109808

    The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial volume Imaris iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. 3D images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.
    Figure Legend Snippet: The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial volume Imaris iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. 3D images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.

    Techniques Used: Activity Assay, Fluorescence, Microscopy, Inhibition, Software, Control, Concentration Assay, MANN-WHITNEY



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    The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial <t>volume</t> <t>Imaris</t> iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. <t>3D</t> images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.
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    The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial volume Imaris iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. 3D images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.

    Journal: iScience

    Article Title: Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission

    doi: 10.1016/j.isci.2024.109808

    Figure Lengend Snippet: The hydrolase activity of F0F1-ATP synthase compensates for the dysfunction of respiratory chain and prevents mitochondrial fission (A) Mitochondrial network observed with MitoTracker Green by fluorescence microscopy. Left panel: representative images showing the different morphologies of the mitochondrial network. Right panel: Colorimetric representation of the mitochondrial network according to mitochondrial volume Imaris iso-surface rendering. (B) Quantification of mitochondrial volume following OXPHOS inhibition. 3D images analyses were performed using Imaris software (Bitplane). The iso-surface module quantifies the volume of mitochondria in μm 3 . Corresponding percentage of mitochondrial volume of ≤1 μm 3 (fragmented, in violet), ≤20 μm 3 (Normal, in green) and >20 μm 3 (connected, in red). n = 3, analyses were performed on 12 images on three independent biological replicates. Results are presented as means ± SEM. ∗ indicates significant difference from control conditions (Vehicle, Veh). (C) Diagram summary under normal conditions reveal that F0F1-ATP synthase and hydrolase activities work simultaneously in mitochondria with a high proportion of forward action. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors. (D) BMS inhibition of F0F1-ATPase on human fibroblasts. Panels: schematic representation of ATP hydrolase activity following drug application. Activity of mitochondrial F0F1-ATPase (V) normalized to citrate synthase (CS). Fibroblasts were treated in culture conditions 4 h with oligomycin or different concentrations of BMS in order to define the effective BMS concentration that inhibits F0F1-ATPase activity. n = 2, each in duplicate. (E and F) Mitochondrial network following BMS treatments observed using MitoTracker Green by fluorescence microscopy in human control cells, treated for 4 h (E) or 6 h (F) with BMS (30 μM), BMS + R, BMS + A, or BMS + O or Veh. n = 5, analyses were performed on 12 images on four independent biological replicates. Results are presented as means ± SEM.∗ p < 0.05, Mann-Whitney U test. Schematic representations of the changes in the activity of the complexes and shape of mitochondria following the presence of inhibitors with BMS or O.

    Article Snippet: 3D images analyses were performed using Imaris software (Bitplane).

    Techniques: Activity Assay, Fluorescence, Microscopy, Inhibition, Software, Control, Concentration Assay, MANN-WHITNEY